Journal: International Journal of Molecular Sciences

Article Title: Moderate Physical Activity as a Prevention Method for Knee Osteoarthritis and the Role of Synoviocytes as Biological Key

doi: 10.3390/ijms20030511

Figure Lengend Snippet: ( A – E ): IL-1β immunohistochemistry. ( A ) IL-1β immunolabeling was very weak in the synovium of Group 1; ( B ) IL-1β immunolabeling was very weak in the synovium of Group 2; ( C ) IL-1β immunolabeling was moderate in the synovium of Group 3; ( D ) IL-1β immunolabeling was weak in the synovial membrane of Group 4; ( E ) Graph representing the densitometric count (Log2 densitometric count − pixel 2 ) of IL-1-immunolabeling identified among groups. For details, see the text. ( F – J ): IL-4 immunohistochemistry. ( F ) IL-4 immunolabeling was moderate in the synovium of Group 1; ( G ) IL-4 immunolabeling was strong in the synovium of Group 2; ( H ) IL-4 immunolabeling was weak in the synovium of Group 3; ( I ) IL-4 immunolabeling was strong in the synovial membrane of Group 4; ( J ) Graph representing the densitometric count (Log2 densitometric count − pixel 2 ) of IL-4-immunolabeling identified among groups. For details, see the text. ( K – O ): IL-6 immunohistochemistry. ( K ) IL-6 immunolabeling was moderate in the synovium of Group 1; ( L ) IL-6 immunolabeling was strong in the synovium of Group 2; ( M ) IL-6 immunolabeling was very strong in the synovium of Group 3; ( N ) IL-6 immunolabeling was very strong in the synovial membrane of Group 4; ( O ) Graph representing the densitometric count (Log2 densitometric count − pixel 2 ) of IL-6-immunolabeling identified among groups. For details, see the text. ( P – T ): IL-10 immunohistochemistry. ( P ) IL-10 immunolabeling was moderate in the synovium of Group 1; ( Q ) IL-10 immunolabeling was strong in the synovium of Group 2; ( R ) IL-10 immunolabeling was weak in the synovium of Group 3; ( S ) IL-10 immunolabeling was strong in the synovial membrane of Group 4; and, ( T ) Graph representing the densitometric count (Log2 densitometric count − pixel 2 ) of IL-10-immunolabeling identified among groups. For details, see the text. In inserts are the image analyses by the software in which red color represents immunolabeling. ( A – D , F – I , K – N , P – S ): Objective lens, 20×; scale bars: 50 µm. Results were presented as the mean ± SD. ANOVA was used to evaluate the significance of the results. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.

Article Snippet: After blocking, the sections were incubated overnight at 4°C with rabbit polyclonal anti-IL-1β (ab9787; Abcam), diluted 1:100 in PBS (Bio-Optica); rabbit polyclonal anti-IL-4 (ab9622; Abcam), diluted 1:100 in PBS (Bio-Optica); goat polyclonal anti-IL-6 antibody (sc-1265; Santa Cruz Biotechnology Inc., Dallas, TX, USA) diluted 1:100 in PBS (Bio-Optica); rabbit polyclonal anti-IL-10 (ab34843; Abcam), diluted 1:100 in PBS (Bio-Optica); mouse monoclonal anti-TNF-α antibody (ab199013; Abcam ) diluted 1:100 in PBS (Bio-Optica); mouse monoclonal anti-MMP-13 antibody (sc-81547; Santa Cruz Biotechnology Inc.) diluted 1:100 in PBS (Bio-Optica); rabbit polyclonal anti-lubricin antibody (ab28484; Abcam), diluted 1:100 in PBS (Bio-Optica).

Techniques: Immunohistochemistry, Immunolabeling, Software

Journal: Poultry Science

Article Title: Research Note: Bovine lactoferrin in chickens: an investigation into its viability as an antibiotic alternative

doi: 10.1016/j.psj.2024.103658

Figure Lengend Snippet: (A) Time plot for jejunum segment with ELISA. (B) Time plot for ileum segment with ELISA. (C) Western blot–after loading the indicated amounts of bovine lactoferrin and ovotransferrin in each lane, immunoblotting was carried out using the previously identified unconjugated polyclonal bLF-specific antibody.

Article Snippet: After blocking with bovine serum albumin ( BSA ), the universal blocking buffer for an hour at room temperature, sections were then incubated overnight at 4°C with goat anti-Bovine Lactoferrin (Bethyl A10-126A), the primary antibody.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot